This book constitutes the refereed proceedings of the Third Latin American Workshop, LAWCN 2021, held in Sao Luis do Maranhao, Brazil, during December 8-10, 2021.The 13 full papers and 3 short papers included in this book were carefully reviewed and selected from 27 submissions. They were organized in topical sections as follows: Interdisciplinary applications of Artificial Intelligence (AI) and Machine Learning (ML); AI and ML applied to robotics; AI and ML applied to biomedical sciences; Health issues and computational neuroscience; Software and hardware implementations in neuroscience; and Neuroengineering - science and technology.

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